Cognomen Holthuis (Holthuis 
) , worldwide, is a rare last name. The cognomen Holthuis is characteristic of Netherlands, where it is quite a rare surname, Germany, where it is a rare surname, and Sweden. In absolute terms, this name is the most popular in Netherlands, Germany, and the United States.
| VARIANT | COUNTRY |
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| Holthuis | Australia, Germany, Sweden, United States, Netherlands |
| k.h. holthuis Dutch architect, NL (b. 1852) |
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lipke holthuis Dutch carcinologist (1921-2008), NL (b. 1921) |
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| w. holthuis NL (b. 1866) |
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| tonnis holthuis Dutch architect, NL (b. 1880) |
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ben holthuis Dutch journalist, NL (b. 1950) |
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| joop holthuis NL (b. 1932) |
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| hans holthuis Dutch prosecutor and judge, NL (b. 1947) |
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| jan holthuis German politician, DE (b. 1950) |
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| piet holthuis NL (b. 1944) |
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| gerard holthuis NL (b. 1952) |
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| gabriele holthuis DE (b. 1958) |
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| lüko holthuis German association football player, DE (b. 1968) |
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| j. holthuis researcher |
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| holthuis j researcher |
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| bernadette holthuis PhD, University of Washington, 1995 |
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